Evaluation of phenotypic methods for the detection of carbapenemases applicable to low complexity laboratories

The spread of carbapenemase-producing gram-negative bacilli is a global public health problem. Several authors have proposed phenotypic assays to presumptively detect these enzymes applicable low and medium complexity laboratories. In the present study, we developed compared different techniques using strains genetically identified as carbapenemase-producing. All tested methods detected presence carbapenemases. carbapenem inactivation method (MIC) modified with without EDTA (mMIC-eMIC) were simplest easiest interpret but their disadvantage was on time required obtain results. direct Carba NP Carba-Blue colourimetric fastest they depend reagent preparation accurate pH adjustment solutions. Synergy discs, boronic acid Triton Hodge Test (THT) require technical expertise evaluate true synergism. Whereas, Disk Carbapenemase (DCT) that presented greatest difficulties.